TY - JOUR A2 - Argyriou, Andreas A. AU - Guo, Kai AU - Song, Lei AU - Chang, Jianyong AU - Cao, Peicheng AU - Liu, Qi PY - 2020 DA - 2020/11/06 TI - AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway SP - 8890452 VL - 2020 AB - Objective. Our study was aimed at investigating the mechanistic consequences of the upregulation of adipocyte enhancer-binding protein 1( AEBP1) in glioblastoma (GBM). Methods. The expression of AEBP1in GBM was assessed by bioinformatics analysis and qRT-PCR; the effects of AEBP1on GBM cell proliferation, migration, invasion, and tumor growth in vitro and in vivo were detected by a CCK-8 assay, colony formation assay, scratch assay, Transwell assay, and subcutaneous tumor formation, respectively. The activation of related signaling pathways was monitored using western blot. Results. Tumor-related databases and bioinformatics analysis revealed that AEBP1was highly expressed in GBM and indicated poor outcome of patients; its high expression that was also confirmed in GBM tissues and cell lines was closely related to the tumor size. The results of in vitro experiments showed that AEBP1could significantly promote GBM cell proliferation, migration, and invasion; in vivo experiments suggested that AEBP1could contribute to the growth of GBM tumors. AEBP1could upregulate the level of IκBαphosphorylation, decrease IκBαexpression, activate the NF- κB signaling pathway, and promote the expression of downstream oncogenes. Conclusion. Upregulated AEBP1“绿带运动”促进“绿带运动”细胞增殖、迁移,and invasion and facilitates tumor growth in vivo by activating the classical NF- κB pathway. SN - 0953-4180 UR - https://doi.org/10.1155/2020/8890452 DO - 10.1155/2020/8890452 JF - Behavioural Neurology PB - Hindawi KW - ER -